Figure 4.

Intracellular ectoine content (heterologous production vs. uptake). Intracellular ectoine concentrations of the recombinant ectoine producers E. coli DH5α pOSM12 (black bars) and pOSM2 (grey bars), the latter supplemented with IPTG for induction of the lac promoter upstream of ectA, and of the control strain E. coli DH5α pHSG575, supplemented with 2 mM ectoine in the growth medium, (white bars) at salinities between 1% and 5% NaCl in minimal medium MM63. Mean values and standard deviations are based on three independent experiments.