Table 1.

Primer and probe sequences used for quantitative real-time PCR for determining copy number of the luciferase gene.

	Luciferase		Barley CONSTANS-like CO2 genea
Primers	LucF1	CCGCCTGAAGTCTCTGATTAAGTAC	HvCon2F1	TGCTAACCGTGTGGCATCAC
	LucR1	GACACCTGCGTCGAAGATGTT	HvCon2R1	GGTACATAGTGCTGCTGCATCTG
Probes	LucP	6FAM-AGGCTATCAGGTGGCTCCCGCTG-TAMRA	HvCon2P	VIC-CATGAGCGTGTGCGTGTCTGCG-TAMRA

^aThe barley CONSTANS-like CO2 gene was used as an internal positive control.