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Expression of RhoA and Rac1 in hESCs and their involvement during human embryo implantation. (A) Immunohistochemistry of frozen tissue sections of midsecretory human endometrium and of cultured hESCs demonstrates the presence of RhoA and Rac1. Endometrial stromal (s) cells and epithelial (e) cells within the tissue are indicated. (B) Toxin B pretreatment of hESCs (0.1 ng/ml, 1 h) before coculture inhibits embryo invasion and trophoblast (T) spreading.
