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Detection of 2G12-like antibodies in HIV-1 patient sera. HIV-1-negative and -positive sera was tested in a competitive binding multiplexed immunoassay to determine whether any component of the antibodies elicited during natural infection could compete with PE-labeled 2G12 for binding to immobilized gp120. In this format, a decrease in median fluorescence intensity indicates the presence of competitive-binding antibodies.
