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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Dev Dyn. 2008 Jul;237(7):1755–1766. doi: 10.1002/dvdy.21590

Figure 4.

Figure 4

Rbmx is necessary for normal neural development independent of neural induction and mesoderm formation. Embryos are either uninjected controls, injected with Rbmx MO in one cell at the 1-cell stage (B,D,F,H,J,L) or one side at the 2-cell stage Injected (N,M,P; injected side to the right). Embryos were analyzed at stage 14 (A–L) or 17 (M–R). (A,B) N-tubulin positive primary neurons do not form in Rbmx MO injected embryos. (C,D) Sox2 expression shows normal morphology of the neural plate. (E–H) early mesoderm formation is normal as judged by MyoD (E,F) and Xbra (G,H) expression. (I,J) Otx5 expression in the anterior neural plate is not excluded from the presumptive forebrain. (K,L) Noggin expression is absent in the anterior neural ridge (red arrow) indicating perturbed anterior neural development. (M,N) Pax2 expressing interneurons in the spinal neural plate fail to form on the injected side. (O,P) Reduced Slug expression on the injected side indicate defect in neural crest formation. (Q,R) medio-lateral patterning of the spinal neural plate proper is normal judged by Pax6 expression.