Table 2. Results of RNA quality control evaluation.
| Experimental group | Scaling factor | Average background | PCall % | β-Actin 3′to 5′ ratio | GAPDH 3′to 5′ ratio |
|---|---|---|---|---|---|
| Infected 1 |
2.5 |
64.5 |
56.9 |
2.6 |
2.7 |
| Infected 2 |
2.5 |
54.1 |
54 |
2 |
2.1 |
| Infected 3 |
2.4 |
55.8 |
52.4 |
1.8 |
1.9 |
| Control 1 |
2.4 |
60.6 |
52.9 |
2.8 |
2.6 |
| Control 2 |
2.4 |
54.9 |
53.7 |
3 |
2.6 |
| Control 3 | 4.6 | 53.7 | 47.2 | 3.1 | 2.8 |
Triplicate samples of five-cornea pools were used from Candida albicans-infected or mock-infected control animals. PCall % is the percent of probe sets that are called “present” by Affymetrix’s algorithm. The 3′ to 5′ ratio is the 3′-end to 5′-end probe intensity ratio. The number of “present” probes for housekeeping probe sets (β-actin and GAPDH) was 3, and the number of spike-in probe sets (BioB, BioC, BioD, and CreX) was 2.