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. 2008 Jun 13;283(24):16693–16701. doi: 10.1074/jbc.M709923200

FIGURE 5.

FIGURE 5.

hCLEC9A is not a phagocytic receptor. A, schematic representation of the chimeric Dectin-1/CLEC9A receptor. B, fluorometric quantitation of zymosan (zym) binding by the transduced RAW264.7 macrophages or NIH3T3 fibroblasts, in the presence or absence of soluble β-glucan (β-glu), as indicated. RFU, relative fluorescence units. Shown are the mean ± S.D. of one representative experiment of three. C, flow cytometric quantitation of zymosan uptake (black histograms) by the chimera or Dectin-1 expressing RAW264.7 macrophages or NIH3T3 fibroblasts, as indicated. Cytochalasin D (gray-filled histograms) was used to inhibit actin polymerization and served as a control in this assay. The histograms shown are representative of at least three independent experiments, and the bars indicate the percentage of cells with internalized particles. *, p < 0.05 versus control (Student's t test). D, representative confocal images demonstrating FITC-zymosan (green) uptake in NIH3T3 fibroblasts transduced with Dectin-1, but not the chimeric receptor. Actin was stained with TRITC-phallodin, and is shown in red.