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. 1997 Oct;8(10):1829–1844. doi: 10.1091/mbc.8.10.1829

Figure 1.

Figure 1

(A) Schematic of Rom2p. (B) Immunoblot analysis of Rom2p::3XHA. (A) The Rom2 protein is predicted to be 1356 amino acids in length and contains a dbl-homology (DH) domain from amino acids 660 to 856 and a pleckstrin homology (PH) domain from amino acids 878 to 984. Epitope tagging of the protein results in the fusion of three tandem copies of the HA epitope to the carboxyl terminus of Rom2p (see MATERIALS AND METHODS). (B) Proteins from total cell extracts of an untagged ROM2 strain (Y1725; HA tag −) and an isogenic ROM2::3XHA strain (Y1723; HA tag +) were separated on an 8% polyacrylamide gel containing SDS. An immunoblot was prepared and probed with 12CA5 anti-HA monoclonal antibody (see MATERIALS AND METHODS). A protein of approximately 165 kDa is detected in extracts from ROM2::3XHA cells but not from ROM2 untagged cells. The mass of molecular weight markers are shown in kilodaltons.