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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Cell Cycle. 2006 Sep 19;5(21):2495–2500. doi: 10.4161/cc.5.21.3423

Figure 2.

Figure 2

Figure 2

Figure 2

S-phase DNA damage checkpoint analysis in G2 synchronized cells. (A) Wild-type cells (yFS104) were synchronized in G2 by centrifugal elutriation, 0.015% MMS or 10 mM HU were added immediately and samples were collected every 20 minutes for flow cytometry. (B) G2 synchronized cultures of wild type (yFS104), rad3Δ (yFS189), adh1:cdc25 (yFS357) and cdc2-Y15F (KGY14) were treated and collected for flow cytometry as in panel A; for clarity, only the MMS treated samples are shown. (C) cdc25Δ cdc2-Y15F (yFS445) cells synchronized in G2, 0.015% or 0.03% MMS or 10 mM HU was added immediately and samples were collected every 20 minutes for flow cytometry.