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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Oncogene. 2008 Jun 16;27(44):5856–5866. doi: 10.1038/onc.2008.195

Figure 2.

Figure 2

Results of gene microarray analysis. a. The number of genes showing significant up- or down-regulation by Runx1, 2 and 3 at 1% and 5% false discovery rate cut-off (Rank Products method (Breitling et al., 2004)). b. Overlap of the Runx transcriptome is shown by the large number of genes significantly regulated by more than one family member. The chart includes 150 high confidence genes regulated by any Runx gene at 1% FDR. Relaxing stringency to 5% and 10% FDR results in inclusion of most of these target genes in a common transcriptome: c. Venn diagram highlighted the filtration strategy for further analysis. Of 69 regulated by all three constitutively expressed Runx genes at 5% FDR, 50 genes were selected on the basis of regulation by an inducible RUNX1-ER™ construct. The gel depicts the molecular weight shift of RUNX1-ER induced in the presence of 4-OHT.