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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Oncogene. 2008 Jun 16;27(44):5856–5866. doi: 10.1038/onc.2008.195

Figure 4.

Figure 4

4a. Kinetics of regulation of selected target genes by RUNX1-ER™. Transcript levels were measured by qt-RT-PCR analysis 1, 2 and 6 hours after induction of the construct with 4-OHT. 4b. Runx2 occupation of promoter regions of Rgs2,C/EBPδ, and Rgc32 in vivo as assessed by ChIP analysis. Specificity was demonstrated by lack of site recognition by a negative control polyclonal antisera, α-GST or when primary antibody was not included in the immunoprecipitation reaction. Primers flanking a previously defined Runx binding site in the osteocalcin gene promoter (Bglap2) were used as a positive control.