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Cloning scheme for cationic ELPs and their ELP fusion proteins showing coding DNA strand, the corresponding amino acid sequence, the ELP guest residue amino acids in bold, and the location of key endonuclease restrictions sites. PflM I and Bgl I restriction enzymes are used for recursive directional ligation to oligomerize ELP monomer genes in (A) as well as for gene excision for subcloning into pET expression vectors at the SfiI site shown in (B).
