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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Biomacromolecules. 2007 Apr 4;8(5):1417–1424. doi: 10.1021/bm060849t

Figure 6.

Figure 6

Yield of (A) Trx-ELP fusion proteins and (B) target Trx protein from 50 mL of E.coli culture as a function of the ELP length (30-36 pentapeptides for the short tags and 64-90 pentapeptides for the long tags) and guest residue composition of the ELP purification tag. Protein yield is reported as the mean±SD (n=3). The gravimetric yield of Trx is higher for the lower molecular weight tag (30-36 pentapeptides) in the case of all three ELP guest residue compositions and the yield of Trx-ELP is greatest for the ELP[KV7F] tags. The poorest fusion protein and target protein yield was exhibited by the ELP[KV2F] proteins suggesting that expression is highly dependent on the guest residue composition and not easily predictable.