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Effects of additional glucose and glycerol on cell density (A), the amount of protein expressed per cell (B), and protein yield per ml of culture (C) in 50 ml uninduced cultures of E. coli expressing GFP-ELP using LB medium with phosphate buffer (control) as a function of time (four replicates total). Supplements used are 20 g glucose/L (glucose), 4 ml glycerol/L (glyercol), 20 g glucose and 4 ml glycerol/L (Gly + Gluc), and 8 ml glycerol/L (2× glycerol).
