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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Biotechnol Prog. 2006;22(3):638–646. doi: 10.1021/bp0503742

Figure 5.

Figure 5

Effect of 20 natural amino acids (denoted by single letter codes) on cell density (A), the amount of protein expressed per cell (B), and protein yield per ml of culture (C) in 50 ml uninduced cultures of E. coli expressing GFP-ELP after 24 hours (two replicates total). Various amino acids (100 mM) are divided into 5 groups according to their initial precursors. Data for various conditions were normalized to the control (Ctl, LB + PBG). For the control, OD600, mean FI/cell and total FI/ml are 11.1, 287.7, and 20932, respectively.