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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: J Hepatol. 2006 Nov 2;46(2):330–339. doi: 10.1016/j.jhep.2006.09.010

Figure 7.

Figure 7

Western Blot Analysis for Bcl-2, Bax, Bcl-XL and Cytochrome c Release (A). 50 μg of liver protein were electrophoresed in either 10% or 12% SDS-PAGE and immunoblotted for Bcl-2, Bax, and Bcl-XL. Twenty-five μg of cytosolic fraction were electrophoresed in a 12% SDS-PAGE and immunoblotted for cytochrome-c. AU of densitometry corrected for β-tubulin expression are indicated under the blots and refer to means±S.E.M. (n=3). ***p<0.001 for CD vs. CH and for CD+W vs. CH+W; •••p<0.001 for CD+W vs. CD. Caspase-3 (B) and Caspase-8 Activity (C). In (B) and (C) the cleaved AFC product was determined by fluorimetry and results are expressed as AU of fluorescence/mg protein and are means±S.E.M. (n=8). **p<0.01 for CD vs. CH and ***p<0.001 for CD+W vs. CH+W; ••p<0.01 for CH+W vs. CH and •••p<0.001 for CD+W vs. CD. The Number of Apoptotic Cells (D) per high power field were determined by TUNEL staining, **p<0.01 for CD vs. CH, ***p<0.001 for CD+W vs. CH+W, and •••p<0.001 for CD+W vs. CD. CH: chow diet, CD: choline-deficient diet, W: whiskey.