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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: Circ Res. 2008 Jul 3;103(4):369–377. doi: 10.1161/CIRCRESAHA.108.174607

Figure 1. Deletion and mutation analysis of the TM promoter region.

Figure 1

A) Deletion and mutation analysis of the TM promoter.

HUVECs were transiently transfected with luciferase reporter constructs with different parts of the human TM 5′-flanking region deleted. Cell were treated with vehicle (V), atorvastatin (A), or pre-treated with mevalonate for 30 minutes before being treated with atorvastatin (M+A) for 24 hours.

Deletion/mutation of HSE1 and HSE3, or of SP1/KLF element partly inhibits statin-induced TM upregulation.

B) Mutation analysis of HSE and/or SP1/KLF element.

HUVECs, transiently transfected with luciferase reporter constructs with HSE3, SP1/KLF, or both elements mutated, were treated with vehicle (V), atorvastatin (A) or pravastatin (P) for 24 hours.

Mutation of either HSE3 or SP1/KLF element only partly inhibited statin-induced TM upregulation, whereas, mutation of both elements together completely abolished statin-induced TM upregulation.

Relative light units (RLU) were determined.

Atorvastatin: 1×10−5M; mevalonate: 5×10−4M; pravastatin: 4×10−5M

* p < 0.05 versus vehicle.