Fig. 1.
Lolal serves as a novel partner for Rib in SG and TR morphogenesis. (A-G) SG defects are observed in rib and lolalK02512,mz mutants. Arrows indicate stage 12 SGs in WT (A), rib alleles (B-E), lolalK02512,mz (F) and rib1/+; lolalK02512,mz (G) mutants. SG phenotypes were divided into three classes: WT (migrated, A), moderate (turned posteriorly but failed to migrate, F), and severe (failed to turn, B-E, G). (H-N) TR defects are observed in rib and lolalK02512,mz mutants. Arrowheads indicate stage 14 DT of metamere 4 in WT (H), rib alleles (I-L), lolalK02512,mz (M) and rib1/+; lolalK02512,mz (N) mutants. DT phenotypes were divided into three classes based on the distance migrated towards metamere 3: WT (greater than two-thirds, H), moderate (between one-and two-thirds, M, N), and severe (less than one-third, I-L). (O-T) An antibody to Rib shows loss of nuclear localization in rib2 mutants and of epithelial nuclear localization in lolalK02512,mz mutants. Rib (green) exhibited robust nuclear localization in WT SG (arrow in O), TR (arrowhead in R), and adjacent mesoderm (asterisk in O,R) and failed to localize to the nuclei of all of these tissues in the rib2 BTB domain mutant (P,S). Rib exhibited weak nuclear localization in lolalK02512,mz mutant SG (Q) and TR (T) but maintained robust nuclear localization in adjacent mesoderm. Embryos are stage 11 for SG and stage 12 for TR. Scale bars are 10 μm. An antibody to Sas marks SG and TR lumena in black (A, H), brown (B-G, I-N), or red (O-T). All embryos are oriented with dorsal up and anterior to the left unless otherwise noted.