Table 2.

Antibodies and antibody dilutions used in this study.

Antibody	Produced in	Dilution*	Reference / Source
Actin	Mouse	1:2000	MP Biomedicals, Inc.
Avl	Chicken	1:500	Lu and Bilder 2005
Crb	Mouse	1:10	DSHB†
Csp	Mouse	1:40	DSHB‡
GFP	Mouse	1:200	Molecular Probes
GFP	Rabbit	1:40000 / 1:10000	Molecular Probes
pMoe	Rabbit	1:500	Karagiosis and Ready, 2004
Rab11	Rabbit	1:500	Satoh et al., 2005
Rib	Rat	1:50	P. Bradley and D. Andrew, unpublished
SAS	Rabbit	1:5000 / 1:500	E. Organ and D. Cavener, unpublished
β-gal	Mouse	1:10000 / 1:500	Promega

^*When appropriate, dilutions used both for HRP and fluorescence stainings are provided, respectively. Otherwise, only the dilution used for fluorescence staining is provided.

^†The monoclonal antibody CQ4 developed by Elisabeth Knust was obtained from the Developmental Studies Hybridoma Bank (DSHB) developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242.

^‡The monoclonal antibody 6D6 developed by Seymour Benzer was also obtained from DSHB. Appropriate secondary antibodies conjugated to biotin (Vector Labs), Alexa Fluor 488, 555, 568, 647, or Rhodamine (Molecular Probes) were used in 1:500 dilution.