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Western blot analysis and detection of dipeptidase activity by cell-based assays. (A) Western blots of cell lysates collected from HEK-vector (column 1), HEK-FAP (column 2) and HT-29 (column 3). (B) Activation of Ac-GPGP-2SBPO in 5 hours. Only HEK-FAP was able to activate the probe. (C) Activation of GPGP-2SBPO in 5 hours. Only HT-29 was able to activate the probe.
