Accuracy of extraction procedures as assessed through quantitative recovery of exogenous corticoids from fecal extracts. a, Slopes of pooled samples extracted with three different protocols and spiked with standards provided with the corticosterone RIA kit. Method 1 (triangles): y = 0.9931x + 0.4752, r2 = 0.9979; method 2 (circles): y = 1.0002x + 0.3133, r2 = 0.993; method 3 (squares): y = 1.1118x + 0.0028, r2 = 0.9993. b, Slopes of pooled samples extracted with two different protocols and spiked with standards provided with the cortisol RIA kit. Method 1 (triangles): y = 1.0886x − 0.0012, r2 = 0.9954; method 3 (squares): y = 1.0556x + 0.0006, r2 = 0.9991.