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. Author manuscript; available in PMC: 2008 Oct 7.

Published in final edited form as: Hepatology. 2008 Oct;48(4):1054–1061. doi: 10.1002/hep.22464

(A) Quantification of HCV RNA. Huh7.5 cells electroporated with either GND RNA or JFH1 RNA were further transfected using the siRNAs indicated under the chart for 48 hrs. Total cellular RNA was then isolated for qRT-PCR analysis. JFH1 cells transfected with the control siRNA were arbitrarily defined as 100%. The results represent the average of at least three independent experiments. The PERK(2) siRNA, which was more efficient in suppressing the expression of PERK was used for the analysis. (B) Western-blot analysis of LC3 and Atg7. Cells in a 10-cm dish transfected by the GND RNA (lanes 1 and 5), JFH1 RNA (lanes 2–4 and 6–7) were further transfected with the control (C) siRNA (lanes 1, 2, 5 and 6), 4.7 μg of LC3 siRNA (lane 3), 9.5 μg of LC3 siRNA (lane 4) or 9.5 μg of Atg7 siRNA (lane 7).

(A) Quantification of HCV RNA. Huh7.5 cells electroporated with either GND RNA or JFH1 RNA were further transfected using the siRNAs indicated under the chart for 48 hrs. Total cellular RNA was then isolated for qRT-PCR analysis. JFH1 cells transfected with the control siRNA were arbitrarily defined as 100%. The results represent the average of at least three independent experiments. The PERK(2) siRNA, which was more efficient in suppressing the expression of PERK was used for the analysis. (B) Western-blot analysis of LC3 and Atg7. Cells in a 10-cm dish transfected by the GND RNA (lanes 1 and 5), JFH1 RNA (lanes 2–4 and 6–7) were further transfected with the control (C) siRNA (lanes 1, 2, 5 and 6), 4.7 μg of LC3 siRNA (lane 3), 9.5 μg of LC3 siRNA (lane 4) or 9.5 μg of Atg7 siRNA (lane 7).