Skip to main content
. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cell Cycle. 2008 May 30;7(15):2384–2391. doi: 10.4161/cc.6363

Figure 3.

Figure 3

lp alleles retain significant functional activity. A) Luciferase activity of H2009 RB(-) cells co-transfected with the pG5-luciferase reporter, wildtype RB (wt) or the indicated low-penetrant RB mutants fused in-frame to a Gal4 binding domain, and either an empty VP16 activation domain (AD) or the AD fused in-frame with MyoD (AD-MyoD) as described in Material and Methods. Cells were incubated at the indicated temperatures for 72 hrs before luciferase double reporter assay measurements. Error bars indicate standard errors from two independent experiments performed in triplicate. B) SaOS2 RB(-) cells were transfected with the indicated RB plasmids and the number of ‘flat cells’ per 10 high power fields (100×) following 2 weeks of G418 selection was determined by manual counting and normalized to the number of ‘flat cells’ 25 induced by wild-type (wt) RB. Error bars indicate standard errors from three independent experiments performed in triplicate.