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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cell Cycle. 2008 May 30;7(15):2384–2391. doi: 10.4161/cc.6363

Figure 4.

Figure 4

Effect of Hsp90 inhibition on R661W stability. A) RB protein immunoblot using lysates from two ECC4 aliquots (lanes 1,2) compared with whole cell extracts from three different lung cancer cell lines with known wildtype (lanes 3,4) or absent RB (H2009, lane 5) expression. B) Immunoblot of ECC4 or control wt RB cells (A549) treated for 16 h with DMSO vehicle or geldanamycin (GA). C) Immunoblot of A549 and ECC4 cells treated with indicated drugs or subjected to heat shock (HS) at 42°C for 30 min followed by 4 h recovery. D) RB (-) cells H2009 cells were transiently transfected with indicated plasmids and subjected to GA and/or velcade (Vcd) exposure for 16 h followed by harvesting and immunoblot analysis. E) Immunoblot of A549 and ECC4 cells incubated in the presence or absence of 100ug/ml CHX and/or 1uM GA for the indicated time points.