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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: J Exp Ther Oncol. 2008;7(2):89–97.

Table 1.

Expression of Fas and VEGF165 in LM7 cells after transfectiona

Cell lines Fas expressionb
(%)
VEGF (ng/OD)c
LM7 14.1 582.5 ± 69.59
LM7/vector 21.6 276.8 ± 22.0d
LM7/Fas 81.1 256.6 ± 26.75d
LM7/Fas/vector 82.5 283.3 ± 31.32d
LM7/VEGF165 21.0 2045.9 ± 48.9e
LM7/Fas/VEGF165 74.1 1297.1 ± 12.3f

Abbreviations: OD = optic density

a

LM7 cells were transfected with empty vector alone, Fas, Fas + empty vector (Fas/vector), VEGF165, or Fas + VEGF165 (Fas/VEGF165).

b

Fas expression was quantified by flow cytometry analysis.

c

VEGF165 protein was measured by ELISA. The value of the VEGF165 protein was adjusted by the OD (MTT assay lived cells in the plate).

d

LM7/vector vs LM7 (level of VEGF), p = 0.0526, LM7/vector vs LM7/Fas (level of VEGF), p = 0.8059, LM7/Fas vs LM7/Fas/vector, p = 0.8130.

e

LM7/VEGF165 vs LM7/vector, P < 0.0001.

f

LM7/Fas/VEGF165 vs LM7/Fas/vector, P < 0.001.