Table 1.
Cell lines | Fas expressionb (%) |
VEGF (ng/OD)c |
---|---|---|
LM7 | 14.1 | 582.5 ± 69.59 |
LM7/vector | 21.6 | 276.8 ± 22.0d |
LM7/Fas | 81.1 | 256.6 ± 26.75d |
LM7/Fas/vector | 82.5 | 283.3 ± 31.32d |
LM7/VEGF165 | 21.0 | 2045.9 ± 48.9e |
LM7/Fas/VEGF165 | 74.1 | 1297.1 ± 12.3f |
Abbreviations: OD = optic density
LM7 cells were transfected with empty vector alone, Fas, Fas + empty vector (Fas/vector), VEGF165, or Fas + VEGF165 (Fas/VEGF165).
Fas expression was quantified by flow cytometry analysis.
VEGF165 protein was measured by ELISA. The value of the VEGF165 protein was adjusted by the OD (MTT assay lived cells in the plate).
LM7/vector vs LM7 (level of VEGF), p = 0.0526, LM7/vector vs LM7/Fas (level of VEGF), p = 0.8059, LM7/Fas vs LM7/Fas/vector, p = 0.8130.
LM7/VEGF165 vs LM7/vector, P < 0.0001.
LM7/Fas/VEGF165 vs LM7/Fas/vector, P < 0.001.