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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Mol Microbiol. 2003 Mar;47(5):1207–1224. doi: 10.1046/j.1365-2958.2003.03366.x

Fig. 2.

Fig. 2

Immuno-electron microscopic detection of AP65 on cryosections on the surface and different sites of high-iron (A1) vs. low-iron grown (B1) trichomonads of Trichomonas vaginalis T068-II. Anti-AP65 serum IgG followed by gold-conjugated goat anti-rabbit IgG was used in this experiment. Large numbers of gold particles were evident within hydrogenosomes of high-iron (A2) parasites. Few particles (arrow) were detectable in the hydrogenosome (H) of low-iron (B2) organisms. Other antisera to adhesins gave similar results. The mAbs F11, F28-1 and DM116 (Fig. 3) to AP65 gave results like those in a2 and b2 in high- and low-iron respectively.