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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Mol Microbiol. 2003 Mar;47(5):1207–1224. doi: 10.1046/j.1365-2958.2003.03366.x

Fig. 4.

Fig. 4

Fluorescence experiments showing detection of AP65 on the surface of non-permeabilized (A) and intracellularly of permeabilized (B) trichomonads of isolate T016 with mAb 12G4 characterized in Fig. 3. The mAbs F11 and L64 to AP65 and to an intracellular protein (Alderete et al., 1987), respectively, were immunoreactive only with permeabilized organisms (B3 and B5). No signal was evident with non-permeabilized trichomonads (A3 and A5). Brightfield photomicrographs of the same fields accompany the fluorescence pictures.