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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Mol Microbiol. 2003 Mar;47(5):1207–1224. doi: 10.1046/j.1365-2958.2003.03366.x

Fig. 6.

Fig. 6

Comparison of two-dimensional patterns of total proteins (A) versus hydrogenosome proteins (B) of surface-iodinated T. vaginalis used in the ligand assay. Immunoblots of proteins after the ligand assay probed with mAbs F11 (AP65) and F5 (AP33) show quantitatively greater amounts of proteins in the total protein extracts (A1) compared with hydrogenosome extracts (B1). Inserts on bottom left of A1 and B1 are the Coomassie brilliant blue stained two-dimensional gels. The multiple bands for AP65 and AP51 are readily visible. The spot with a high pI at the lower right side corresponds to AP33, as seen in Fig. 5. Parts A2 and B2 present autoradiograms of gels of iodinated proteins of total trichomonad and hydrogenosome (B2) extracts, respectively, used in the ligand assay. No labelled proteins were evident for the hydrogenosome two-dimensional patterns even with prolonged exposure to X-ray film.