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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Mol Microbiol. 2003 Mar;47(5):1207–1224. doi: 10.1046/j.1365-2958.2003.03366.x

Fig. 7.

Fig. 7

Contact with host cells shows increased amounts of surface-expressed adhesins. In this experiment, high iron trichomonads were first incubated with monolayers of immortalized AO VECs before removing and iodinating (see Experimental procedures). Part A shows the same amount of proteins enriched by the ligand assay, as evidenced by the equal intensity of Coomassie brilliant blue stained gels. Autoradiograms in part B illustrate a greater intensity of signal of the surface-labelled proteins of trichomonads after contact with the host cells. Equivalent parasite numbers were used in both samples, which were handled identically. Identical results were obtained with MS74 VECs.