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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Brief Funct Genomic Proteomic. 2008 Jul 16;7(4):303–311. doi: 10.1093/bfgp/eln034

Figure 1.

Figure 1

Schematic representation of rAAV production for gene therapy applications using the baculovirus-insect cell system. The insect cell is infected by three BEVs containing the rep, cap genes and the vector genome. After infection, the Rep 78 and Rep 52 proteins are expressed and the vector genome is rescued and replicated independently of the baculovirus DNA. Concurrently, the capsid proteins VP1, VP2, and VP3 are expressed and assembled into virus-like particles (VLP). The vector genome is packaged into the VLPs and producing the biologically active rAAV particle.