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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Brief Funct Genomic Proteomic. 2008 Jul 16;7(4):303–311. doi: 10.1093/bfgp/eln034

Figure 3.

Figure 3

Characterization of rAAV using isopycnic CsCl gradient, DNA concentration, and biological activity. Gradient fractions (1 ml) were analyzed for: a) Presence of rAAV capsid proteins (VP1, VP2 and VP3) by polyacrylamide gel electrophoresis and western blot with anti-AAV capsid anti-serum and b) Concentration of vector genomes expressed as vector genomes (vg)/ml determined by qPCR. The biological activity or transduction units (tu)/ml determined by treating HEK 293 cells with dilutions of rAAV that express green fluorescent protein (GFP) and measuring the GFP positive cells using flow cytometry. The values of vg/ml and tu/ml are graphed for the CsCl isopycnic fractions. The peak activity values were obtained with densities corresponding to full capsids of rAAV (F). The position of the F and empty capsids (E) across the CsCl density gradient (1.41gcm3 and 1.37g/cm3, respectively) are indicated. The corresponding fraction numbers are indicated in each panel.