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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Mol Microbiol. 2004 Aug;53(4):1099–1108. doi: 10.1111/j.1365-2958.2004.04192.x

Fig. 3.

Fig. 3

Immunoblots with specific mAb DM116 detecting the amount of AP65 in total protein preparations (A) and bound to HeLa cells in a ligand assay (B) from equal numbers of S and AS transfected trichomonads compared to control Wt organisms.

A. Total proteins from 107 trichomonads were subjected to SDS-PAGE on 10% acrylamide before blotting onto Hybond-P membranes. As a control to show equivalent protein amounts in each lane, the blot was also probed with mAb F5 to the AP33 adhesin (Engbring and Alderete, 1998a; 1998b).

B. AP65 bound to HeLa cells were solubilized and electrophoresed for blotting and probing with mAb DM116 as in part A. Total proteins and the ligand assay were as described in Experimental procedures.