Fig. 4.

Effect of growth of trichomonads in low- (centre, hatched bars) versus high-iron (solid black bars) medium on ecto-5′-nucleotidase activity among representative fresh Trichomonas vaginalis isolates and the long-term-grown 347 isolate. Different iron conditions were compared with normal TYM-serum medium. Bars represent the mean±S.D. for at least 3 experiments. Results were analysed statistically by Student’s t-test (P<0·05). Statistically significant enzyme activity differences when compared to control are indicated by an asterisk.