Figure 6.

Western blot analysis of Bcl-2 family protein expression. After neutrophils were co-cultured with T. vaginalis for 5, 10 and 20 h, cell lysates were electrophoresed in 12% SDS-PAGE and electroblotted on nitrocellulose membranes. Blots were incubated with primary and horseradish peroxidase-conjugated secondary antibodies and then developed using an enhanced chemiluminescence detection kit. Representative Western blots of three separate experiments are shown.