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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Cancer Res. 2008 Jul 15;68(14):5716–5723. doi: 10.1158/0008-5472.CAN-08-0001

Figure 5. Effect of Jagged-1 knockdown on cell proliferation in ovarian cancer cells.

Figure 5

A. Expression of Jagged-1 and Notch3 mRNA in IOSE cells (IOSE7, IOSE10 and IOSE29) and ovarian cancer cell lines (TOV21G, A2780, OVCAR3, SKOV3, MPSC1 and ES2) was measured by quantitative RT-PCR. TOV21G and OVCAR3 showed highest levels of Jagged-1 expression. B. Jagged-1 shRNA does not have a significant effect in cell growth at both day 3 and day 5 in IOSE 7 and IOSE10 cells. C. Jagged-1 shRNA significantly inhibits cell growth in ovarian cancer cell lines which over-expresses Jagged-1. p<0.01 (day 3); p<0.001 (day 5), Student’s t test. D. Colony formation is significantly reduced in both OVCAR3 and TOV21G cells transfection with Jagged-1 shRNA, comparing to vector-transfected group (p<0.01, Student’s t test). Cells were seeded at both low and high density and cell colonies were measure at 2 weeks after plating.