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. Author manuscript; available in PMC: 2009 Aug 5.
Published in final edited form as: Vaccine. 2008 Jun 13;26(33):4150–4159. doi: 10.1016/j.vaccine.2008.05.075

Figure 4. Growth analysis of parental SLE and chimeric viruses in Vero cells or in human neuroblastoma cells following incubation at different temperatures.

Figure 4

An efficiency of plaque formation assay was performed with the indicated viruses in Vero cells and SH-SY5Y cells. Confluent monolayers of cells were infected with serial ten-fold dilutions of virus at 32°C, overlaid with semisolid growth media, and then incubated for five days at 32, 35, 36, 37, 38, or 39°C. Plaques were visualized by immunostaining and quantitated. The limit of detection (100.7 PFU/ml) is indicated by a dashed line.