Table 1.

Mutations identified after Vero cell passage of SLE/DEN4 viruses

Virus	C/prM junction	Clone	Gene	Nucleotide position	Nucleotide substitution	Amino acid changea
SLE/DEN4	GGTR	549	E	1162	U→C	Ile70→Thr
			E	1420	U→C	Phe156→Ser
			E	1506	A→G	Thr185→Ala
			NS4B	7196b	A→C	Leu112→Phe
			3′ UTR	10341	U→C	-
SLE/DEN4	GGTR	551	NS4B	7196b	A→C	Leu112→Phe
SLE/DEN4	TSGR	554	E	1162	U→C	Ile70→Thr
			E	1362	U→C	Tyr137→His
			E	1420	U→C	Phe156→Ser
			NS3	4710	G→A	Val52→Met
			NS4B	7174b	C→U	Thr105→Ile
SLE/DEN4Δ30	GGTR	545	prM	588	A→G	Lys46→Glu
			NS4B	7196b	A→C	Leu112→Phe
SLE/DEN4Δ30	GGTR	546	E	1162	U→C	Ile70→Thr
			NS1	2827	U→C	Phe124→Ser
			NS3	5857	U→C	Leu434→Pro
			NS4B	7196 b	A→C	Leu112→Phe
SLE/DEN4Δ30	TSGR	609	E	1162	U→C	Ile70→Thr
			NS3	4879	A→C	His108→Pro
			NS4B	7174 b	C→U	Thr105→Ile
SLE/DEN4-436,437	GGTR	41	E	1140	G→U	Ala63→Ser
			E	1420	U→C	Phe156→Ser
			NS4B	7196b	A→C	Leu112→Phe
SLE/DEN4-436,437	GGTR	42	NS4B	7196b	A→C	Leu112→Phe
SLE/DEN4-654,655	GGTR	46	NS2B	4367	A→G	Ile67→Met
			NS4B	7196b	A→C	Leu112→Phe

^aNumbering indicates amino acid position within protein. Only coding changes and nucleotide substitutions within the UTRs are indicated in this table.

^bVero cell adaptation mutations (non-adventitious) that were incorporated into the cDNA clone are indicated in bold.