Figure 10.

Synthesis rates of 220aa-Hac1p encoded by intron-less and intron-containing mRNAs. The hac1Δ strain (KMY1145) was transformed with a vector alone (V) or a mutant version (Δintron 220-type or Ala221Stop) of the HAC1 gene. Each transformant was grown and pulse-labeled for 5 min with [³⁵S]methionine and [³⁵S]cysteine as described in MATERIALS AND METHODS. Hac1p and Pdi1p were immunoprecipitated and subjected to SDS-PAGE (12% gel). Radioactive bands were visualized by using a BioImaging Analyzer BAS-2000. The positions of molecular mass markers, 220aa-Hac1p, and Pdi1p are indicated in kilodaltons.