Fig. 2.

The 17 nucleotide Wee1 region exerts translational regulation on a heterologous reporter mRNA. (A) The indicated reporter mRNAs were injected into immature oocytes and cultured in the presence (P) or absence (I) of progesterone for 16 h, and polyadenylation of the reporter mRNAs was assessed by northern blot (left panel). The time course of polyadenylation of β-globin/Wee17 reporter mRNAwas assessed in immature (Imm) or progesterone-stimulated (Prog) at the indicated times (hours) by northern blot (right panel). In this experiment, GVBD₅₀ occurred at 5 h. (B) Immature oocytes were injected with the indicated GST reporter mRNAs and time matched immature (I) and progesterone-stimulated (P) samples analyzed for GST protein accumulation by western blot after 15 h of culture.