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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2008 May 3;45(5):578–584. doi: 10.1016/j.freeradbiomed.2008.04.036

Figure 1. Generation of Fe(II)NO and NO/HNO by HRP-mediated peroxidation of NH2OH.

Figure 1

A) EPR spectra of HRP (50 or 500 μM) in the presence of NH2OH (100 mM) and H2O2 (100 mM). The spectrum shows the characteristic three line spectrum of a ferrous nitrosyl heme complex. B) Chemiluminescence signal intensity during peroxidation of NH2OH by HRP. H2O2 (final concentration 5 μM) was injected (arrows) into a sealed glass reaction chamber containing 10 mM phosphate buffer (pH 7.4, 2 mL) with DTPA (50 μM), NH2OH (500 μM) and HRP (5 μM) in either the absence or presence of GSH (100 μM) as indicated.