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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Hypertension. 2008 Jun 9;52(2):256–263. doi: 10.1161/HYPERTENSIONAHA.108.112706

Figure 3.

Figure 3

(A) Representative immunohistochemical staining for F4/80-positive cells (macrophages) in mice infused for 2 weeks with either vehicle or Ang II. Reddish-brown color in the cytoplasm indicates positive staining. Positive staining for macrophages was found mainly in the tubulointerstitial space (arrows) and to a lesser extent in the glomerulus (arrow-heads). Scale Bar = 50 μm.

(B) Quantitative analysis of immunohistochemical staining for F4/80-positive cells (macrophages) at 2 (left) and 4 weeks (right). In CCR2+/+ with Ang II-induced hypertension the number of positive cells increased significantly at both 2 and 4 weeks (p < 0.01, vehicle vs Ang II). However, macrophage infiltration was higher at 2 weeks. In CCR2−/− with Ang II induced hypertension, macrophage infiltration did not increase at either 2 or 4 weeks. (2 weeks, n=5 per group; and 4 weeks, n=8 per group).