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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6306–6312. doi: 10.1158/0008-5472.CAN-08-0870

Figure 3.

Figure 3

Detection of the HA tagged EGFP-NGEP mutants by immunofluorescence. The 293T cells were transfected with the HA tagged insertions mutants and 24 hr post-transfection, the cell were processed for immunofluorescence in both nonpermeabilized and permeabilized conditions. The expression of the proteins was detected by using anti-HA MAb and the signal was detected using Alexa-546 labeled goat anti-mouse antibody. The tagged mutants were then categorized according to their apparent intracellular (A) or extracellular (B) localizations.