. Author manuscript; available in PMC: 2009 Aug 1.

Published in final edited form as: Drug Metab Dispos. 2008 May 7;36(8):1637–1649. doi: 10.1124/dmd.107.019562

Table 1.

Estimated kinetic parameters for the formation of 2,3-DiOHCBZ by cDNA-expressed human P450 enzymes and human liver microsomes.

Enzyme	Mean Specific P450Content^a	Per Cent of Total Hepatic P450^a	2,3-DiOHCBZ formation
Enzyme	Mean Specific P450Content^a	Per Cent of Total Hepatic P450^a	S₅₀^b,^c	n^b	V_max^b	Cl_int	Abundance Normalized Cl_int^g	Percentage Normalized Cl_in
CYP2C19	19	4	30 ± 3	1.13 ± 0.09	2.11 ± 0.09 ^d	0.045 ^f	0.90	22
CYP3A4	108	20	203 ± 85	0.87 ± 0.10	2.93 ± 0.51 ^d	0.029 ^f	3.13	78

Pooled HLM	N.A.	N.A.	257 ± 76	1.22 ± 0.15	190 ± 32 ^e	0.218^g	N.D.	N.D.

Mean specific content of P450 or per cent of total P450 in human liver microsomes (Rodrigues, 1999),

Cooperativity coefficient (value ± S.D),

μM,

pmol/pmol P450/min,

pmol/mg protein/min,

μl/pmol P450/min, and

μl/mg protein/min. HLM, human liver microsomes. N.A., not available. N.D., not determined.