Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics* | Source or reference |
|---|---|---|
| H. hepaticus | ||
| 51449 | Parent strain for all H. hepaticus strains | ATCC† |
| hypA mutant | hypA::ery; Err | This study |
| hypB mutant | hypB::ery; Err | This study |
| hypC mutant | hypC::ery; Err | This study |
| ureE mutant | ureE::ery; Err | This study |
| ureG mutant | ureG::ery; Err | This study |
| ΔureG mutant | ΔDureG::ery; Err | This study |
| hh0809 mutant | hh0809::ery; Err | This study |
| E. coli | ||
| TOP10 | Cloning strain | Invitrogen |
| Plasmids | ||
| pGEM-T | Cloning vector; Apr | Promega |
| pBC-KS | Cloning vector; Cmr | Stratagene |
| pBluescript-KS | Cloning vector; Apr | Stratagene |
| pSLB167 | pBC-KS with ery (EcoRV); Cmr Err; source of ery cassette | Mehta et al. (2007) |
| pGEM-hypA | pGEM with a 760 bp sequence containing H. hepaticus hypA and part of hh0809 | This study |
| pSLB164 | pGEM-hypA with ery gene inserted within NruI of hypA | This study |
| pGEM-hypB | pGEM with a 814 bp sequence containing H. hepaticus hypB | This study |
| pSLB165 | pGEM-hypB with ery gene inserted within HindIII of hypB | This study |
| pGEM-ureE | pGEM with a 734 bp sequence containing H. hepaticus ureE | This study |
| pSLB166 | pGEM-ureE with ery gene inserted within HindIII of ureE | This study |
| pBC-ureG | pBC-KS with a 904 bp sequence containing H. hepaticus ureG | This study |
| pSLB173 | pBC-ureG with ery gene inserted within AvaII of ureG | This study |
| pGEM-ureG | pGEM with a 904 bp H. hepaticus ureG with an engineered HindIII | This study |
| pSLB175 | pGEM-ureG with ery gene inserted within HindIII of ureG | This study |
| pSLB290 | pGEM-hypA deleted for MluI (PstI/NsiI-digested; religated) | This study |
| pSLB293 | pSLB290 with ery inserted within MluI of hh0809 | This study |
*
Apr, Ampicillin resistance; Cmr, chloramphenicol resistance; Err, erythromycin resistance.
†
ATCC, American Type Culture Collection, Manassas, VA, USA.