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. Author manuscript; available in PMC: 2008 Oct 7.
Published in final edited form as: Vision Res. 2007 Jan 30;47(5):624–633. doi: 10.1016/j.visres.2006.11.020

Figure 6.

Figure 6

Apoptosis in P18 mouse retinas. Paraffin sections of P18 C57 wild type (A, C) and rd10 (B, D) mouse eyes were subjected to fluorescent TUNEL (A, B) or fluorescent immunochemistry using an antibody specific to activated caspase-3 (C, D). Sections were counterstained with propidium iodide. TUNEL-positive and activated caspase-3-positive cells fluoresced yellow-green. Wild type sections showed almost no TUNEL signal (A), whereas rd10 sections had abundant TUNEL signal in the outer nuclear layer and occasionally inner segments of photoreceptor cells, but not in other layers of the retina (B). Wild type sections showed almost no activated caspase-3 immunofluorescence (C), whereas rd10 sections had abundant signal in the inner segments of photoreceptor cells, but not in other layers of the retina (D). Control stainings lacking primary antibody had no signal (data not shown).