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. Author manuscript; available in PMC: 2009 Mar 25.
Published in final edited form as: Oncogene. 2008 Jun 2;27(43):5729–5740. doi: 10.1038/onc.2008.180

Fig. 1.

Fig. 1

(a) Protein expression of wt and mutant Rheb in CEF. CEF transfected with recombinant RCAS encoding wt Rheb or Q64L were lysed 10 days post transfection. The lysates were separated on a 4-20% gradient SDS/PAGE gel and transferred to a polyvinylidene difluoride membrane. The membrane was probed with anti-FLAG and anti-tubulin antibody. (b) Focus formation induced by mutant Rheb. CEF were infected with the RCAS vector or recombinant RCAS viruses expressing wt Rheb, Q64L or N153T at different dilutions as indicated. The cultures were overlaid with nutrient agar and stained with crystal violet on day 14. (c) Anchorage-independent colony formation induced by mutant Rheb. CEF were transfected with the RCAS vector or recombinant RCAS viruses expressing wt Rheb, Q64L or N153T, then seeded in nutrient agar 12 days post transfection and photographed on day 10 after seeding.