Skip to main content
. Author manuscript; available in PMC: 2009 Jul 23.
Published in final edited form as: J Am Chem Soc. 2008 Jun 25;130(29):9268–9281. doi: 10.1021/ja801602q

Figure 2.

Figure 2

pSUPAR3 plasmid. Plasmid contains two copies of the amino-acyl tRNA synthetase specific for the unnatural amino acid (Uaa-RS), one under the control of a constitutively-on glnS promoter and the second under the control of an arabinose-inducible ara promoter. The plasmid also encodes three copies of the unnatural amino acid specific amber tRNA with a proK promoter, a p15A origin and a chloramphenicol CmR resistance marker.