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. Author manuscript; available in PMC: 2009 Jul 11.
Published in final edited form as: J Mol Biol. 2008 May 3;380(3):465–475. doi: 10.1016/j.jmb.2008.04.065

Figure 2.

Figure 2

(A) Cryo-EM of sucrose gradient–purified 80α procapsids. Inset, 2× magnified view of one procapsid with dimensions of the shell and the inner core indicated. (B) Cryo-EM of CsCl-purified 80α virions. The 2 nm spacing of the internal DNA is clearly visible. One thin-walled, empty capsid is also shown (inset). (C) Negatively stained CsCl-purified 80α procapsid fraction, containing a mixture of procapsids, tails and procapsids with attached tails. (D) Cryo-EM of sucrose gradient-purified SaPI1 procapsids. Inset, one of the about 5% 80α-size procapsids found in the SaPI1 procapsid sample. Scale bar, 100 nm.