Skip to main content
. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: J Pharmacol Exp Ther. 2008 Apr 25;326(1):286–295. doi: 10.1124/jpet.108.139675

Figure 7.

Figure 7

Effect of SoRI 20040, SoRI 20041, and SoRI 2827 on the dissociation of [125I]-RTI55 binding from hDAT. Membranes were incubated with 0.01 nM [125I]-RTI55 for 2 hours at 25°C. At this point, baseline samples were filtered, and then RTI 55 (1 μM) was added to samples 2 and 4. Ten minutes later, test drug was added to samples 3 and 4 to generate the following conditions: control (no addition, sample 1), RTI-55 (1 μM, sample 2), test drug (10 μM, sample 3), and RTI55 (1 μM) + test drug (10 μM, sample 4). For the purpose of data analysis, the 100% of control point was “time 0 min” for conditions 1 and 2, and “time 10 min” for conditions 3 and 4. Samples were then filtered at the indicated time points. The data of conditions 2 and 4 were fit to a one component dissociation model and the data of condition 3 was fit to a two component dissociation model (see Tables 2 and 3). Each point is mean ± SD(n=3).