. Author manuscript; available in PMC: 2009 Jul 1.

Published in final edited form as: J Pharmacol Exp Ther. 2008 Apr 25;326(1):286–295. doi: 10.1124/jpet.108.139675

Table 2.

Experimental Design for Kinetic Dissociation Experiments

Condition	1^st Addition (Time 0)	2^nd Addition (Time 10 min)
1. Control	none	None
2. RTI-55	RTI-55 (1μM)	None
3. Test Drug	none	Test Drug: SoRI-20040 (10 μM) or SoRI-20041 (10 μM) or SoRI-2827 (10 μM)
4. RTI-55 + Test Drug	RTI-55 (1μM)	Test Drug: SoRI-20040 (10 μM) or SoRI-20041 (10 μM) or SoRI-2827 (10 μM)

Binding of [¹²⁵I]RTI-55 (0.01 nM) to hDAT proceeded for 2 hr at 25° C. At this point (Time 0), RTI-55 (1 μM) was added to conditions 2 and 4. Ten minutes later, test drugs were added to conditions 3 and 4. For the purpose of data analysis, the 100% of control point was “time 0 min” for conditions 1 and 2, and “time 10 min” for conditions 3 and 4. Each data point is the mean±SD of three experiments. The data were fit to a one (condition 1, 2 4) or two-component (condition 3) exponential decay model using KaleidaGraph version 3.6 to calculate the kinetic constants.